Viability Test of the Gourami Scales Collagen Extract (Osphronemus Goramy) on the Human Gingival Fibroblast Cells

Abstract

Background: Periodontal disease is a pathological inflammatory condition of the periodontal tissues
which surround the teeth, including Human Gingival Fibroblasts (HGF). HGF regeneration is through
the accelerating proliferation of tissue engineering therapy needs. Generally, the tissue engineering uses
regenerative materials from cow or pig as the therapies, but these materials have some flaws. Thereby,
this research aims to find the alternative materials regenerative tissue engineering scaffold collagen type
1 derived from the gourami fish scales. This research is also conducted to test the viability of fish scales
collagen gourami against the Human Gingival Fibroblasts for 24 hours.
Objective: To determine the concentration of fish scale collagen gourami which can maintain the viability
of human gingival fibroblast cells for 24 hours.
Method: HGF is taken from the healthy gingiva and planted in 96 well plates. Fish scales collagen gourami
with a concentration of 0.32 mg/ml, 0.16 mg/ml, 0.04 mg/ml, 0.02 mg/ml and 0.01 mg/ml were added to
each well and incubated during 24 hours. MTT Assay is performed to see the viability of fibroblast cells.
Results: The viability of HGF were increased after the addition of fish scales collagen gourami on the
concentration 0.32 mg/ml until 0.01 mg/ml. The viability of the cells after the addition of fish scale collagen
gourami was shown above 100%.
Conclusion: Fish scales collagen gourami has the potential in tissue engineering and the concentration of
0.01 mg/ml shows the highest viability of HGF.