Effects of E. Coli Infection on Kidney Function Tests in Experimentally Inoculated Rats

Abstract

The aim of this study was to evaluate the kidney function tests in rats experimentally infected with E. coli
with monitoring signs of UTI. Forty female rats were divided into two groups, infected (20 rats) and control
(20 rats). The infected group were inoculated intraurethral by 108 suspension of E. coli. The animals were
monitored for presence of signs. E. coli urine bacterial demonstration were done at 1 week before infection
and 24hrs., 48hrs, 96hrs, 6 days, 12 days and 24 days after infection. Blood samples were collected at zero,
24hrs, 3 days, 6 days, 14 days and 24 days. Blood samples for serum BUN and creatinine were collected.
The findings suggested that the E.coli isolation has been continuous 24 days since experimentation started.
After 24 hrs. post infection, infected rats were suffering from UTIs sings, including frequent urination, pain
on urination, foul smelling, diarrhea, poor appetite, and fever. Also, the current results showed that at zero
time, all rats present normal value of serum urea and creatinine, Post-infection with E. coli, A significant
increase in BUN and creatinine was shown by the infected group at 24 hours, 3 days, 6 days, 14 days and 24
days, as opposed to the control group.
In conclusion, E. coli can induce UTI in rats manifested by increase BUN and Creatinine.