Genotyping Diversity of Pseudomonas aeruginosa Isolates, Isolated from Baquba City

Abstract

This research aims to assess the similarities and variances between MDR Pseudomonas aeruginosa clinical
isolates from different infections using MLST molecular typing technique in Baquba city. One hundredeighty clinical samples were collected from urinary tract, wounds and burns infections, included (110)
urine, (50) wounds and (20) burns. Twenty isolates (11.11%) of Pseudomonas aeruginosa were diagnosed
based on microscopic, morphological, biochemical tests and confirmed by VITEK-2 system. Antibiotic
susceptibility test for the twenty isolates was performed against six antibiotics using disk diffusion method,
the results showed (100%) resistance for Cephalothin, Ceftazdim (60%), Colistin (35%), Amikacin (25%),
Ciprofloxacin (20%), while (0%) resistance for Imipenem. The results also showed that 16 (80%) of isolates
were produced extended spectrum beta-lactamase (ES?L) and did not show their production for Metallobeta-lactamase (M?Ls). Biofilm was detected by Micro-Titer Plate method and the result showed that all the
isolates (100%) were biofilm producers. Multi-locus sequence typing (MLST) was used because broadness
and ease of access of MLST database. Seven housekeeping genes (acsA, aroE, guaA, mutL, nuoD, ppsA,
andtrpE) were sequenced and the results were compared with MLST database and allele profile for each
isolate was used to determine sequence types (STs).New Iraqi bacterial strain has been identified and
recorded in database of MLST (PubMLST) under the name (AS-85U).