In Vitro Study for Nano Hydroxyl Apatite and Chitosan Coated Surface after Immersion in Simulated Body Fluid

Abstract

Multiple attempts are made to develop coating layers on the implant surface to enhance ossiointegration and
support implant functions. The mixture of chitosan with HA prepared by sol–gel process to make organic–
inorganic hybrid composite that has the ability to make network with or without covalent bonds between
components.
In this study the immersion of titanium disc coated via sol – gel technique by nano-HA and chitosan in
simulated body fluid.
Materials and Method: Composite of nano hydroxy apatite/chitosan was prepared by sol – gel technique
after preparation of two solutions of calcium nitride and phosphorous pentaoxide, then the magnetic stirrer
was used for mixing of these solutions, then 3 mg of chitosan were added to the mixture and the mixture
was stirrer for 15 hours to complete the mixing. Dip coated of titanium samples, then the samples were
removed and dried by hot air oven, finally samples were sintered at 4000
C for one hour. Coated samples
were immersed in SBF in closed containers and placed in incubators at temperature 370
C for 30 days.
Analysis of the surface include thickness measurement, X-ray diffraction (XRD), scanning electron
microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDX).
The results of thickness measurement showed increase in thickness of the coated layer after immersion in
SBF compared with thickness before immersion. The results of XRD showed the appearance of new peaks
of HA, SEM showed there are additional accumulation of HA granules on the original coating layer. EDX
test showed decreased in the concentration of the main ions component (Ca, Na, P, O and K) when compared
with coated samples before immersion in SBF.