Bacteriological and Molecular Study of Klebsiella Pneumoniae Isolated from Patients with Urinary Tract Infections from Several Hospitals in Baghdad

Abstract

This study obtained 20 isolates of Klebsiella pneumoniae from out of 50 isolates collected from patients with
urinary tract infection; all isoates were diagnosed and then the sensitivity test for isolates was performed
using the discs method (Kirby-Bauer) to determine the resistance of K.pneumoniae to 10 antibiotics. The
results detected that the highest resistance was against Ampicillin, Cefotaxim, and Piperacillin by 100% for
each of them, while the rest of the antibiotics had a resistance percentage of 80% for Trimethoprim, 54%
for Gentamicin and 65% for Azithromicin, however the lowest resistance was found against Imipenem,
Chloramphenicol and Ofloxacin by 4%, 12. % and 35% respectively. The genomic bacterial DNA of studied
isolates was extracted and the genotyped was performed identify the genetic relationships between the
bacterial isolates using ERIC-PCR method. The results of the study showed the presence of 12 genotypes
and also the results revealed the presence of two clones, each of which contains similar genotypes, while the
rest of the isolates contain different genotypes.