A Comparative Study of DNA Extraction Method with and without Using Proteinase K Enzyme

Authors

  • Ishwar Prasad Dubey1,2, R.K. Kumawat3,4, Shivani Dixit2,3, Kamlesh Kaitholia2 , I.P. Tripathi1 , Divya Shrivastava3 , Pankaj Shrivastava2

DOI:

https://doi.org/10.37506/mlu.v20i4.2152

Keywords:

DNA Extraction, Proteinase k, Forensic, DNA Fingerprinting.

Abstract

Forensic DNA fingerprinting is the most believed method of the criminal investigation. With the advancement
in technology, the method has become more and more sensitive and the technology now uses advanced
method and new generation faster multiplex amplification systems. Most of these improvements in the
technique are after the extraction of DNA. Despite automation in DNA extraction, still, the basic process of
lysis of cells is an integral part of the analysis. We present here the comparison of DNA extraction results
with and without using the proteinase k enzyme.

Author Biography

  • Ishwar Prasad Dubey1,2, R.K. Kumawat3,4, Shivani Dixit2,3, Kamlesh Kaitholia2 , I.P. Tripathi1 , Divya Shrivastava3 , Pankaj Shrivastava2

    1
    Faculty of Science and Environment, MGCGVV, Chitrakoot, Satna-485334, M.P, India, 2DNA Fingerprinting
    Unit, State Forensic Science Laboratory, Sagar-470001, M.P, India, 3School of Life Sciences, Jaipur National
    University, Jaipur-302017, Rajasthan, India, 4DNA division, State Forensic Science Laboratory, Rajasthan,
    Jaipur-302016, India

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Published

2020-11-18

Issue

Vol. 20 No. 4 (2020): Medico-Legal Update

Section

Articles

How to Cite

A Comparative Study of DNA Extraction Method with and without Using Proteinase K Enzyme. (2020). Medico Legal Update, 20(4), 2087-2091. https://doi.org/10.37506/mlu.v20i4.2152