Prevalence and Molecular Detection of Rotavirus in Children in Ramadi City-Iraq
DOI:
https://doi.org/10.37506/mlu.v20i4.2154Keywords:
Molecular detection, RT-PCR, Rotavirus, children patients.Abstract
Matter: This is the first study in Al-Anbar governorate with this design aim to the detection of rotavirus
group A (ROVA).
Method: A total of (150) stool samples obtained from children <5 years with acute gastroenteritis were
randomly collected from Maternity and Children at al-Anbar governorate from (1- 9-2019 to 1-2-2020);
Rotavirus was detected by rapid test for stool samples and real-time polymerase chain reaction for blood
and serum.
Results: Out of total 150 stool sample there were 101(67.3%), 49(32.6%) Negative and positive result
respectively, from rapid test. The result of RT-PCR from serum & blood were Thirty-one (31)81.57% of
(38) samples positive for rotavirus & eight samples were no ct there was Significant value (0.0016)(0.0075)
between PCR (blood, serum) test and Rapid test under p<0.05. Based on that RT-PCR was more sensitive
& specific than rapid test
