Molecular Study of spy1258 gene in Streptococcus Pyogenes Isolated from Pharyngitis Patients in Fallujah City

Abstract

Background : Streptococcus pyogenes (GAS) strict human pathogen that give rise to a wide range of diseases. GAS is the most current bacterial cause of pharyngitis and firstly effect school-age children 5-15years of age. SPY1258 gene is a specialized gene group in GAS and its considered specific for GAS and can be used for its detection as a markers.
Method : A total of 300 throat swab were collected from patients suffering from pharyngitis referred to Fallujah general teaching Hospital, Fallujah taeching Hospital for women and chiladren and Amerya general Hospital , Iraq during a peroid between 2018-2019.
Theses samples were submit for bacteriology and molecvular tests.
Results : Out of all samples screened 43 isolates (14.3)were identified as ß – hemolytic streptococci and only 13 isolates were detected as streptococcus pyogenes by bacteriology and biochemical test and also by PCR GAS isolates exposed in order to detect the particular gene (SPY1258). Antibiotic sensetivity test showed a high incidence of bacterial resistance to macrolids.
Conclusion : Bacitracin sensetive is non- specific test for the identification of Streptococcus pyogenes, and should be used further test, for best result should confirmed by SPY1258 gene. The SPY1258 gene can be used for accurate molecular diagnosis of Streptococcus pyogenes .