Detection of blaCTX-M gene in Klebsiella sp. Causing UTIs in Al-Yarmouk Teaching Hospital

Abstract

Background : Klebsiella sp. causes a wide range of bacterial diseases like pneumonia, UTI and sepsis. Therefore, this study was done to assess the prevalence and molecular characteristics of Klebsiella sp. in 50 samples of men and women isolated from Iraqi patients which was suffering from urinary tract infection (UTI) compared with (10) of healthy individuals. All samples were collected from Al-Yarmouk teaching hospital/Baghdad during January – March 2019. Identification of bacterial isolated were done by using manual culturalprocedures and VITEK 2automated (bioMérieux, France)system, while antibiotic sensitivity test of Klebsiella sp. was achieved by Kirby-Bauer and MIC testing was madeby VITEK 2. The creation of ESBL was phenotypically distinguished by twofold plate cooperative energy test as assigned by to the CLSI rules. Location of bla-quality encoded CTX-M was seen by customary P.C.R system. Out of fifty separates ofK. Pneumonia,26%were planted generation of ESBL utilizing CDT, the MIC utilized withdiverse anti-infection agents in this investigation, 13(26%) of secludes by utilizing VITEK2 AST-GN30 which exhibited that, all disconnects were totally impervious to every one of ceftazidime, cefepime and ceftriaxone with MIC(?16-?64) ?g/ml,and the outcome demonstrated that 61.53% separated of ESBL producion were delicate carbapenem with MIC (?0.25) ?g/ml. Furthermore, PCR examine uncovered that 4(30.76)%of the ESBL creating isolatesharbouredblaCTX-M gene.