A Study on Resveratrol on the Antioxidative and Whitening Cosmeceutical Ingredients

Abstract

Objectives: Resveratrol is a variety of physiological active substances and is an excellent ingredient in
antioxidant, anti-inflammatory and anti-aging action. Therefore, I would like to find out the possibility of
adding cosmetics.
Method: The cytotoxicity test using MTT solution was carried out to check the safety of reverse control. Also
DPPH assay was performed to measure antioxidant activity in resveratrol itself, and ROS was measured to
measure antioxidant activity in cells. NO production was measured to observe anti-inflammatory action, and
tyrosinase activity measurement and melanin production were measured to measure skin whitening activity.
Findings: we were able to confirm the safety of resveratrol’s cytotoxicity. At concentration of 1, 10, 100
?g/mL of Resveratrol, the DPPH radical scavenging activity was observed to show high concentration -
dependent activity of free radical scavenging. And In order to check the antioxidant properties within the
cell, the ROS (reactive oxygen specifications) using DCF-DA were observed to reduce the effects. The
addition of resveratrol 1, 10, 100 ?g/mL on RAW 264.7 macrophages stimulated with lipopolysaccharide
resulted in NO produce inhibition in concentration dependent and a strong inhibition rate of 53% at 100 ?g/
mL concentration. Tyrosinase active action was found to inhibited dose dependant. Melanin produce was
also prevented by dose dependant.
Applications: These results suggest that the active oxygen - induced skin inflammation process is delayed
as much as possible, so that it can be utilized as a functional cosmetic material having an effect on skin
diseases and skin aging.